Polymerase chain reaction–based method for the typing of F18 fimbriae and distribution of F18 fimbrial subtypes among porcine Shiga toxin–encoding Escherichia coli in Germany
نویسندگان
چکیده
منابع مشابه
Frequency of virulence factors in Escherichia coli isolated from pigs with postweaning diarrhea and edema disease in North Carolina
Polymerase chain reaction for five fimbriae (K88, K99, 987P, F41, F18), two heatstable enterotoxins (STa, STb), one heatlabile enterotoxin (LT), and Shiga-like toxin type II (SLT) was performed on Escherichia coli isolates to determine their genotype among isolates from weaned pigs with diarrhea and/or edema disease in North Carolina. A total of 175 isolates were tested. All but two isolates po...
متن کاملNanobody Mediated Inhibition of Attachment of F18 Fimbriae Expressing Escherichia coli
Post-weaning diarrhea and edema disease caused by F18 fimbriated E. coli are important diseases in newly weaned piglets and lead to severe production losses in farming industry. Protective treatments against these infections have thus far limited efficacy. In this study we generated nanobodies directed against the lectin domain of the F18 fimbrial adhesin FedF and showed in an in vitro adherenc...
متن کاملGenome sequences and phylogenetic analysis of K88- and F18-positive porcine enterotoxigenic Escherichia coli.
Porcine enterotoxigenic Escherichia coli (ETEC) continues to result in major morbidity and mortality in the swine industry via postweaning diarrhea. The key virulence factors of ETEC strains, their serotypes, and their fimbrial components have been well studied. However, most studies to date have focused on plasmid-encoded traits related to colonization and toxin production, and the chromosomal...
متن کاملMapping the binding domain of the F18 fimbrial adhesin.
F18 fimbrial Esherichia coli strains are associated with porcine postweaning diarrhea and pig edema disease. Recently, the FedF subunit was identified as the adhesin of the F18 fimbriae. In this study, adhesion domains of FedF were further studied by constructing deletions within the fedF gene and expressing FedF proteins with deletions either together with the other F18 fimbrial subunits or as...
متن کاملDifferentiation of F18ab+ from F18ac+ Escherichia coli by single-strand conformational polymorphism analysis of the major fimbrial subunit gene (fedA).
Toxin-producing Escherichia coli expressing F18 fimbriae colonizes the small intestines of weaned pigs and causes diarrhea, edema disease, or both. The F18 family is composed of two antigenic variants, F18ab and F18ac. Because many strains do not express F18 fimbriae in vitro, identification and differentiation of these two variants are difficult. Single-strand conformational polymorphism (SSCP...
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ژورنال
عنوان ژورنال: Journal of Veterinary Diagnostic Investigation
سال: 2011
ISSN: 1040-6387,1943-4936
DOI: 10.1177/1040638711403417